禽免疫抑制病创新团队

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团队成员

李 凯

1、个人简历

李凯,男,博士,副研究员,硕士研究生导师,禽免疫抑制病创新团队,中国免疫学会会员,主要从事禽免疫抑制病和禽肿瘤病的流行病学、致病机理、感染与免疫机制以及新型基因工程疫苗研究。先后主持和参与国家自然科学基金项目、国家科技支撑计划项目、黑龙江省自然科学基金项目、中法蔡元培交流合作项目、中国博士后科学基金项目等国家和省部级科研项目10余项,目前以第一作者发表SCI论文16篇,获得国家发明专利3项。参与指导博士研究生2名,硕士研究生4名。

 

2、联系方式    

1)研究方向:动物疫苗与分子免疫学

2)电话:15776765828

3)邮箱:likai01@caas.cn

4)所在单位:万博体育max

5)通讯地址:黑龙江省哈尔滨市香坊区哈平路678

 

3、教育经历

2004.09-2008.06  新疆农业大学   动物医学专业   学士

2012.11-2013.11  法国国家食品、环境及劳动卫生署   预防兽医学专业   博士

2008.09-2014.06  中国农业科学院研究生院   预防兽医学专业   博士

 

4、工作经历

2014.07-2016.12  中国农科院哈尔滨兽医研究所   助理研究员

2017.01-至今       中国农科院哈尔滨兽医研究所   副研究员

 

5、承担项目

  1.国家自然科学基金青年项目:利用MDV载体递呈靶向ALV-JCRISPR/Cas9系统预防MDVALV-J感染的研究(316001272017.01-2019.12),20万元;

  2.黑龙江省科学基金项目:利用CRISPR/Cas9技术预防禽网状内皮组织增生病病毒感染的研究(QC20160422016.07-2019.07),5万元;

  3.中国博士后科学基金面上项目:MDV载体递呈靶向REVCRISPR/Cas9系统的研究(2015M5801572014.10-2016.10),8万元;

  4.中央科研院所基本科研业务费:马立克氏病病毒疫苗株多片段拯救系统的建立及活载体疫苗研究(16103020150042015.01-2017.12),21.8万元;

  5.国家自然科学基金面上项目:鸡马立克氏病毒遗传进化及变异毒株特性分析(316701552017.01-2020.12),60万元;

  6.国家科技支撑计划:禽流感等重要家禽疫病流行毒疫苗研究与开发(2015BAD12B032015.01-2019.12),84.25万元。

 

6、代表论文与著作

  1.Li K, Liu Y, Liu C, Gao L, Gao Y, Zhang Y, Cui H, Qi X, Zhong L, Wang X. Evaluation of two strains of Marek's disease virus serotype 1 for the development of recombinant vaccines against very virulent infectious bursal disease virus. Antiviral Res. 2017, 139:153-160.

  2.Li K, Liu Y, Zhang Y, Gao L, Liu C, Cui H, Qi X, Gao Y, Zhong L, Wang X. Protective efficacy of a novel recombinant Marek's disease virus vector vaccine against infectious bursal disease in chickens with or without maternal antibodies. Vet Immunol Immunopathol. 2017, 186:55-59.

  3.Li K, Liu Y, Liu C, Gao L, Zhang Y, Cui H, Gao Y, Qi X, Zhong L, Wang X. Recombinant Marek's disease virus type 1 provides full protection against very virulent Marek's and infectious bursal disease viruses in chickens. Sci Rep. 2016, 6: 39263.

  4.Li K, Liu Y, Liu C, Gao L, Zhang Y, Gao Y, Cui H, Qi X, Zhong L, Wang X. Effects of different promoters on the protective efficacy of recombinant Marek's  disease virus type 1 expressing the VP2 gene of infectious bursal disease virus.  Vaccine. 2016, 34(47): 5744-5750.

  5.Li K, Courtillon C, Guionie O, Allée C, Amelot M, Qi X, Gao Y, Wang X, Eterradossi N. Genetic, antigenic and pathogenic characterization of four infectious bursal disease virus isolates from China suggests continued evolution  of very virulent viruses. Infect Genet Evol. 2015, 30: 120-7.

  6.Li K, Gao L, Gao H, Qi X, Gao Y, Qin L, Wang Y, Wang X. Recombinant infectious bursal disease virus expressing Newcastle disease virus (NDV) neutralizing epitope confers partial protection against virulent NDV challenge in chickens. Antiviral Res. 2014, 101:1-11.

  7.Li K, Gao L, Gao H, Qi X, Gao Y, Qin L, Wang Y, Wang X. Protection of chickens against reticuloendotheliosis virus infection by DNA vaccination. Vet Microbiol.  2013, 166(1-2):59-67.

  8.Li K, Gao L, Gao H, Qi X, Gao Y, Qin L, Wang Y, Wang X. Codon optimization and woodchuck hepatitis virus posttranscriptional regulatory element enhance the immune responses of DNA vaccines against infectious bursal disease virus in chickens. Virus Res. 2013, 175(2):120-7.

  9.Li K, Gao H, Gao L, Qi X, Gao Y, Qin L, Wang Y, Wang X. Enhancement of humoral and cellular immunity in chickens against reticuloendotheliosis virus by DNA prime-protein boost vaccination. Vaccine. 2013, 31(15):1944-9.

  10.Li K, Gao H, Gao L, Qi X, Gao Y, Qin L, Wang Y, Wang X. Adjuvant effects of interleukin-18 in DNA vaccination against infectious bursal disease virus in chickens. Vaccine. 2013, 31(14):1799-805.

  11.Li K, Gao H, Gao L, Qi X, Gao Y, Qin L, Wang Y, Wang X. Recombinant gp90 protein expressed in Pichia pastoris induces a protective immune response against reticuloendotheliosis virus in chickens. Vaccine. 2012, 30(13):2273-81.

  12.Li K, Gao H, Gao L, Qi X, Gao Y, Qin L, Wang Y, Wang X. Development of an indirect ELISA for serological detection of reticuloendotheliosis virus using the gp90 protein expressed in Pichia pastoris. J Virol Methods. 2012, 180(1-2):43-8.

  13.Li K, Gao H, Gao L, Qi X, Qin L, Gao Y, Xu Y, Wang X. Development of TaqMan real-time PCR assay for detection and quantitation of reticuloendotheliosis virus. J Virol Methods. 2012, 179(2):402-8.

 

​7、专利和标准

  1.李凯,刘长军,王笑梅,张艳萍,高玉龙,崔红玉,高立,马立克氏病病毒弱毒疫苗814株的反向遗传操作系统及其在病毒拯救中的应用,201510200990. 8

  2.李凯,王笑梅,刘长军,张艳萍,高玉龙,崔红玉,高立,马立克氏病病毒感染性重组克隆系统及其构建方法和应用,201510304703.8

  3.王笑梅,李凯,刘长军,张艳萍,崔红玉,祁小乐,高立,高玉龙,王永强,高宏雷,表达传染性法氏囊病毒VP2基因的重组鸡马立克氏病病毒疫苗株及其构建方法和应用,20161014733 9.3

  4.李凯,高玉龙,王笑梅,刘长军,张艳萍,崔红玉,高立,祁小乐,王永强,表达J亚群禽白血病病毒GagEnv基因的重组鸡马立克氏病病毒疫苗株及其构建方法和应用,20161 0147336.X

 

(2019年6月更新)

 

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